GFP Antibody, HRP 130-091-833 from Miltenyi Biotec

Product Specs
ItemGFP Antibody, HRP
CompanyMiltenyi Biotec
Price Pricing Info Supplier Page View Company Product Page
Catalog Number130-091-833
Antibody TypePrimary antibodies, Epitope tag antibodies
Clonalitymonoclonal
TargetGFP
Quantity100 µL
Conjugate/TagHRP
ApplicationsWestern blotting, ELISA
FormatReagents are supplied in an aqueous saline solution, pH 6.5 − 7.5, and contain 0.02% methylisothiazolone (MIT) and 0.02% bromonitrodioxane (BND) as preservatives
Reactivityany
IsotypeIgG1
CloneGG4-2C2.12.10
Hostmouse
Research AreaFor research use only
Regulatory StatusFor research use only

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Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) Molecular basis of TASL recruitment by the peptide/histidine transporter 1, PHT1Nature CommunicationsSeptember 14, 2023Tânia F. Custódio, Maxime Killer, Dingquan Yu, Virginia Puente, Daniel P. Teufel, Alexander Pautsch, Gisela Schnapp, Marc Grundl, Jan Kosinski, Christian Löwsubjected to a western blotting assay using an anti-GFP-HRP antibody (Miltenyi Biotec, catalog number 130-091-833) with a 1:4000 dilution for TASL detection or using Strep-tactin-HRP (IBA, catalog number 2-1502-001)

(2) LEAFY homeostasis is regulated via ubiquitin-dependent degradation and sequestration in cytoplasmic condensatesiScienceJune 16, 2023Ulla Dolde, Fernando Muzzopappa, Charlotte Delesalle, Julie Neveu, Fabian Erdel, Grégory Vertusing the following antibodies: Monoclonal anti-GFP horseradish peroxidase-coupled (Miltenyi Biotech 130-091-833, 1/5,000), anti-ubiquitin P4D1 (Cell Signaling, 1/2,000). Experiments were done in triplicates. Rubisco

(3) Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclustersNature communicationsJune 6, 2023Nishi W, Wakamatsu E, Machiyama H, Matsushima R, Saito K, Yoshida Y, Nishikawa T, Takehara T, Toyota H, Furuhata M, et al.(MBL International, D153-11, RRID:AB_2893312) were blotted with anti-GFP (1:5000, Miltenyi Biotec, 130-091-833, RRID:AB_247003), anti-mSHP1 (1:500, Santa Cruz Biotechnology, sc-287, RRID:AB_2173829), anti-mSHP2

(4) The exocyst complex is an essential component of the mammalian constitutive secretory pathwayJournal of Cell BiologyMay 1, 2023Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlickused for Western blot in this study: mouse anti-GFP HRP conjugate antibody (GG4-2C2.12.10; 1:5,000; 130-091-833; Miltenyi Biotec), rabbit anti-EXOC1 antibody (1:5,000; ab118798; Abcam), rabbit anti-EXOC3 antibody

(5) The receptor kinase FERONIA regulates phosphatidylserine localization at the cell surface to modulate ROP signalingScience AdvancesApril 7, 2023Marija Smokvarska, Vincent Bayle, Lilly Maneta-Peyret, Laetitia Fouillen, Arthur Poitout, Armelle Dongois, Jean-Bernard Fiche, Julien Gronnier, José Garcia, Herman Höfte, et al.2 mM leupeptin, 1 mM phenylmethylsulfonyl fluoride, and 5 mM dithiothreitol]. α-GFP-HRP (Miltenyi, 130-091-833) antibody for Western blot was diluted in blocking solution (1% bovine serum albumin in 0.1% Tween 20

(6) Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulatorNature PlantsJanuary 12, 2023Peng Wang, Wei Siao, Xiuyang Zhao, Deepanksha Arora, Ren Wang, Dominique Eeckhout, Jelle Van Leene, Rahul Kumar, Anaxi Houbaert, Nancy De Winne, et al.were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000) (ChromoTek, α-RFP antibody [6G6]), α-AP2S antibody85 (1:500), α-AP2A antibody86

(7) Abscisic acid regulates stomatal production by imprinting a SnRK2 kinase-mediated phosphocode on the master regulator SPEECHLESSScience advancesOctober 7, 2022Yang X, Gavya S L, Zhou Z, Urano D, Lau OS.Technology, 71D10, 1:3000 dilution), monoclonal, HRP-conjugated, anti-GFP antibody (Miltenyi Biotec, 130-091-833, 1:3000 dilution), monoclonal anti–α-tubulin antibody (Santa Cruz Biotechnology, sc-5286 HRP, 1:1000),

(8) The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plantsNature communicationsAugust 1, 2022Platre MP, Satbhai SB, Brent L, Gleason MF, Cao M, Grison M, Glavier M, Zhang L, Gaillochet C, Goeschl C, et al.protein levels detected using anti-GFP horseradish peroxidase-coupled antibodies (Miltenyi Biotech 130-091-833, 1/5,000). Loading control was obtained using the stain-free that allows visualization of proteins in

(9) The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNACell ReportsApril 12, 2022Thibaut Hacquard et al.RRID:AB_871937" Anti Flag-M2-HRP,Sigma-Aldrich,"Cat# A8592, RRID:AB_439702" Anti GFP-HRP,Miltenyi,"Cat# 130-091-833, RRID:AB_247003" Mouse monoclonal anti-GFP (JL8),Clontech (Takara),"Cat# 632381, RRID:AB_2313808" peroxidase-conjugated

(10) Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machineryEMBO reportsApril 5, 2022Zhao H, Sun J, Insinna C, Lu Q, Wang Z, Nagashima K, Stauffer J, Andresson T, Specht S, Perera S, et al.anti‐Ccdc108 (homemade; 1:1,000), and horseradish peroxidase (HRP)‐conjugated mouse anti‐GFP (Miltenyi Biotec; 130‐091‐833; 1:3,000) and rat anti‐HA (Sigma; 12013819001; 1:3,000). The following secondary antibodies were used:

(11) RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactinNature communicationsMarch 21, 2022Keren-Kaplan T, Sarić A, Ghosh S, Williamson CD, Jia R, Li Y, Bonifacino JS.AB_2246059, mouse,1:200, Millipore-Sigma), Streptavidin-HRP (Cat# 21130, 1:10,000, Pierce), GFP-HRP (Cat# 130-091-833, RRID:AB_247003 mouse, 1:2,000, Miltenyi Biotec), LAMTOR4 (C7orf59) (D4P6O) (Cat# 13140, RRID:AB_2798129,

(12) Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasisNature CommunicationsDecember 1, 2021Martina K. Ried et al.1 : 10,000, Calbiochem). For GFP detection, membranes were incubated overnight with an anti-GFP-HRP antibody (130-091-833, dilution 1 : 1000, Miltenyi Biotec). For FLAG detection, membranes were incubated overnight with an

(13) Chaperone-like protein DAY plays critical roles in photomorphogenesisNature CommunicationsDecember 1, 2021Ho-Seok Lee et al.(A8592, T6199, RPN1236, and M1321). Anti-GFP (1:1000) antibody was purchased from Miltenyi Biotec (130-091-833). Native DAY antibody, X-Q94C78-C (1:500), was purchased from AB-mart (http://www.ab-mart.com). X-Q94C78-C

(14) Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 bindingNature CommunicationsDecember 1, 2021Stuart Sullivan, Thomas Waksman, Dimitra Paliogianni, Louise Henderson, Melanie Lütkemeyer, Noriyuki Suetsugu, John M. ChristieMerck 11867423001) anti-GFP-HFP monoclonal antibody (1:5000, clone GG4-2C2.12.10, Miltenyi Biotech 130-091-833), anti-thiophosphoester monoclonal antibody (1:10,000, clone 51-8, Abcam ab133473), anti-UGPase antibody

(15) Warm temperature triggers JOX and ST2A-mediated jasmonate catabolism to promote plant growthNature CommunicationsDecember 1, 2021Tingting Zhu, Cornelia Herrfurth, Mingming Xin, Tatyana Savchenko, Ivo Feussner, Alain Goossens, Ive De Smet(Sigma-Aldrich, USA)). For immunodetection, anti-GFP-HRP antibody (MACS, Miltenyi Biotec, catalog number: 130-091-833) at 1:5000 was used to incubate membranes for 1 h at room temperature. The proteins were detected by

(16) SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulumNature communicationsJuly 27, 2021Saric A, Freeman SA, Williamson CD, Jarnik M, Guardia CM, Fernandopulle MS, Gershlick DC, Bonifacino JS.(Cat#A301-055A, Bethyl Laboratories, Montgomery, TX, USA), mouse anti-GFP HRP-conjugated used at 1:5,000 (Cat# 130-091-833, Miltenyi Biotec, Bergisch Gladbach, Germany). The following secondary antibodies, purchased from ThermoFisher Scientific

(17) Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP poolsScientific reportsJune 23, 2021Kychygina A, Dall'Osto M, Allen JAM, Cadoret JC, Piras V, Pickett HA, Crabbe L.mouse mono LAP2alpha,SAB4200238 SIGMA mouse mono V5,"13202 Cell signaling, rabbit poly" GFP-HRP,130-091-833 Miltenyi m6Adenine,202003 SYSY rabbit poly POT1,ab47082 Abcam rabbit poly TPP1,ab57595 Abcam mouse

(18) ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1Current biology : CBFebruary 8, 2021Keren-Kaplan T, Bonifacino JS.Biotechnology, Santa Cruz, CA), c-Myc-HRP (9E10, Santa Cruz Biotechnology), HA (AB3254, Sigma-Aldrich), GFP-HRP (130-091-833, Miltenyi Biotec, Bergisch Gladbach, Germany), KIF5B (ab167429, Abcam, Cambridge, UK), KLC2 (a mixture

(19) Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root GravitropismCurrent BiologyJanuary 11, 2021Maria Mar Marquès-Bueno et al.12CA5,Sigma-Aldrich,Cat#11583816001; RRID: AB_514505 Mouse monoclonal anti-GFP HRP clone GG4-2C2.12.10,Miltenyi Biotec,Cat#130-091-833; RRID: AB_247003 Rat monoclonal anti-HA High Affinity clone 3F10,Sigma-Aldrich,Cat#11867423001; RRID:

(20) SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistanceNature CommunicationsDecember 1, 2020Andrej Hurný et al.supernatants were separated by SDS-PAGE and subjected to immunoblotting using anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000) and Anti-HA-Peroxidase, (3F10, Roche, dilution 1:5000). Bimolecular

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

GFP Antibody, HRP from Miltenyi Biotec

Miltenyi Biotec

Miltenyi Biotec

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Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

GFP Antibody, HRP from Miltenyi Biotec

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